CDC42 ELISA Kit

Catalog No. ABIN6951508

Product Details CDC42 ELISA Kit

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human CDC42 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for CDC42

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Alternative Name: CDC42 (CDC42 Products)

Synonyms: CDC42Hs ELISA Kit, G25K ELISA Kit, AI747189 ELISA Kit, AU018915 ELISA Kit, cdc42a ELISA Kit, wu:fb07d08 ELISA Kit, wu:fj62g06 ELISA Kit, zgc:63918 ELISA Kit, Xcdc42 ELISA Kit, cdc42hs ELISA Kit, g25k ELISA Kit, CDC42 ELISA Kit, CG12530 ELISA Kit, Cdc42Dm ELISA Kit, D-CDC42 ELISA Kit, D-Cdc42 ELISA Kit, D-cdc42 ELISA Kit, DCDC42 ELISA Kit, DCdc42 ELISA Kit, Dcdc42 ELISA Kit, Dm Cdc42 ELISA Kit, DmCDC42 ELISA Kit, Dmcdc42 ELISA Kit, Dmel\\CG12530 ELISA Kit, cdc42 ELISA Kit, cell division cycle 42 ELISA Kit, Cell division control protein 42 homolog ELISA Kit, cell division control protein 42 homolog ELISA Kit, cell division cycle 42 L homeolog ELISA Kit, CG12530 gene product from transcript CG12530-RC ELISA Kit, Rho family GTPase ELISA Kit, CDC42 ELISA Kit, Cdc42 ELISA Kit, cdc42 ELISA Kit, LOC100636735 ELISA Kit, LOC100637699 ELISA Kit, LOC100638834 ELISA Kit, cdc42.L ELISA Kit

Gene ID: 998

UniProt: P60953

Pathways: MAPK Signaling, Microtubule Dynamics, RTK Signaling, WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling