FCGR2B ELISA Kit

Catalog No. ABIN6951585

Product Details FCGR2B ELISA Kit

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Rat Fc gamma RIIB/CD32b ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for FCGR2B

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Alternative Name: Fc gamma RIIB (CD32b (FCGR2B Products)

Synonyms: CD32 ELISA Kit, CD32B ELISA Kit, FCG2 ELISA Kit, FCGR2 ELISA Kit, IGFR2 ELISA Kit, FCGR2B ELISA Kit, AI528646 ELISA Kit, F630109E10Rik ELISA Kit, Fc[g]RII ELISA Kit, FcgRII ELISA Kit, Fcgr2 ELISA Kit, Fcgr2a ELISA Kit, Fcr-2 ELISA Kit, Fcr-3 ELISA Kit, Ly-17 ELISA Kit, Ly-m20 ELISA Kit, LyM-1 ELISA Kit, Lym-1 ELISA Kit, FCRII ELISA Kit, FcRII ELISA Kit, Fc fragment of IgG receptor IIb ELISA Kit, Fc fragment of IgG, low affinity IIb, receptor (CD32) ELISA Kit, Fc receptor, IgG, low affinity IIb ELISA Kit, low affinity immunoglobulin gamma Fc region receptor II ELISA Kit, FCGR2B ELISA Kit, Fcgr2b ELISA Kit, LOC100856270 ELISA Kit

Gene ID: 289211

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling