FAS ELISA Kit

Catalog No. ABIN7830644

This Colorimetric ELISA kit is designed for the quantitative measurement of Human FAS.

Quick Overview for FAS ELISA Kit (ABIN7830644)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details FAS ELISA Kit

Purpose: FAS (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FAS expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: FAS

Background:

Synonyms: ALPS1A|APO-1|APT1|CD95|FAS1|FASTM|TNFRSF6

Gene Description: Fas (TNF receptor superfamily, member 6)

Gene ID: 355

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity