MAP2K3 ELISA Kit

Catalog No. ABIN7830766

Human MAP2K3 ELISA Kit, Colorimetric assay for quantification of Human MAP2K3.

Quick Overview for MAP2K3 ELISA Kit (ABIN7830766)

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details MAP2K3 ELISA Kit

Purpose: MAP2K3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MAP2K3 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for MAP2K3

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Alternative Name: MAP2K3

Background:

Synonyms: MAPKK3|MEK3|MKK3|PRKMK3

Gene Description: mitogen-activated protein kinase kinase 3

Gene ID: 5606

UniProt: P46734

Pathways: MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2