IL-6 ELISA Kit

Catalog No. ABIN7832831

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-6.

Quick Overview for IL-6 ELISA Kit (ABIN7832831)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Plasma, Serum

Grade: QuickTest

Product Details IL-6 ELISA Kit

Purpose: FastELISA IL-6 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human IL-6 (Interleukin 6) in serum, plasma.

Analytical Method: Quantitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C

Storage Comment: Store at 2-8C for 6 months.Avoid repeated freezing and thawing.

Expiry Date: 6 months

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome