c-MET ELISA Kit

Catalog No. ABIN8055957

This Colorimetric ELISA kit is designed for the quantitative measurement of Dog c-MET.

Quick Overview for c-MET ELISA Kit (ABIN8055957)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Reactivity: Dog

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156.0 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Product Details c-MET ELISA Kit

Minimum Detection Limit: 156.0 pg/mL

Purpose: Sandwich Canine HGFR ELISA Kit to quantitate Canine HGFR in cell culture supernatants, serum, plasma(heparin, EDTA).

Analytical Method: Quantitative

Specificity:

Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 20 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 7.9%, 3.5%, 5.3%
Inter-Assay Precision (CV%): 5.4%, 5.7%, 7.2%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

Target Details for c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: MET

Background:

Background: Hepatocyte growth factor receptor (HGF receptor) is a protein that in humans is encoded by the MET gene. Enables hepatocyte growth factor receptor activity. Involved in cell surface receptor signaling pathway, modulation of chemical synaptic transmission, and positive chemotaxis. Acts upstream of or within several processes, including hepatocyte growth factor receptor signaling pathway, regulation of cytokine production, and striated muscle cell differentiation. Located in membrane. Is expressed in several structures, including alimentary system, branchial arch, central nervous system, genitourinary system, and limb.

Gene Full Name: Hepatocyte growth factor receptor

Gene ID: 403438

UniProt: Q75ZY9

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor