VEGFR2/CD309 ELISA Kit

Catalog No. ABIN8071280

Human VEGFR2/CD309 ELISA Kit, Colorimetric assay for quantification of Human VEGFR2/CD309.

Quick Overview for VEGFR2/CD309 ELISA Kit (ABIN8071280)

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Binding Specificity: pTyr1175, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details VEGFR2/CD309 ELISA Kit

Purpose: Phospho-VEGFR2 (Y1175) and Total VEGFR2 ELISA Kit is a convenient and sensitive assay that can monitor the activation or function of important biological pathways in human cell lysates.

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human vascular endothelial growth factor receptor 2 phosphorylated at site Tyrosine-1175.

Components:

• Pre-Coated 96-well Strip Microplate
• Positive Control
• Anti-Phospho Detection Antibody
• Anti-Pan Detection Antibody (*not included in phospho-only version)
• HRP-Conjugated Secondary Antibody
• HRP-Streptavidin (*not included in phospho-only version)
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Shaker
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare the positive control or sample dilutions

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and positive control as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Target Details for VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Alternative Name: VEGFR2

Background:

Gene Symbols: KDR, FLK1, VEGFR2

Protein Name (Synonyms): Vascular endothelial growth factor receptor 2 (VEGFR-2), Fetal liver kinase 1 (FLK-1), Kinase insert domain receptor (KDR), Protein-tyrosine kinase receptor flk-1

Gene ID: 3791

UniProt: P35968

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling