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Bcl-2 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse and Rat Bcl-2.
Catalog No. ABIN8075129
$812.88
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for Bcl-2 ELISA Kit (ABIN8075129)

Target

See all Bcl-2 (BCL2) ELISA Kits
Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Binding Specificity

pSer70

Reactivity

  • 23
  • 17
  • 15
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
Mouse, Rat

Detection Method

Colorimetric

Method Type

Cell ELISA

Application

ELISA

Sample Type

Cell Culture Cells
  • Purpose

    Phospho-BCL2 (Ser70) Colorimetric Cell-Based ELISA Kit for detecting Phospho-BCL2 (Ser70) in researchers plate their cell line of choice.

    Analytical Method

    Qualitative

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Assay Time

    4 - 5 h

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    6 months
  • Target See all Bcl-2 (BCL2) ELISA Kits

    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

    Alternative Name

    BCL2

    Background

    Gene Aliases: Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, BCL2

    Gene ID

    596

    UniProt

    P10415

    Pathways

    MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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