BRAF ELISA Kit

Catalog No. ABIN8075145

The Human BRAF ELISA Kit (ABIN8075145) is a Colorimetric ELISA Kit designed to quantify Human BRAF.

Quick Overview for BRAF ELISA Kit (ABIN8075145)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details BRAF ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Purpose: Human BRAF ELISA Kit for detecting BRAF in serum, plasma

Analytical Method: Quantitative

Sensitivity: 0.19 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 3 - 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 2-8°C for 12 months, Long term: see manual.

Expiry Date: 12 months

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF

Background: Gene Aliases: B-Raf, proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1, serine/threonine-protein kinase B-Raf, BRAF, B-RAF1, BRAF1, NS7, RAFB1

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy