p300 ELISA Kit
Quick Overview for p300 ELISA Kit (ABIN8078340)
Target
See all p300 (EP300) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- p300 Colorimetric Cell-Based ELISA Kit for detecting p300 in researchers plate their cell line of choice.
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Analytical Method
- Qualitative
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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E1A Binding Protein P300 (EP300) ELISA Kit
EP300 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
E1A Binding Protein P300 (EP300) ELISA KitEP300 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
E1A Binding Protein P300 (EP300) ELISA KitEP300 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
E1A Binding Protein P300 (EP300) ELISA KitEP300 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
E1A Binding Protein P300 (EP300) ELISA KitEP300 Reactivity: Rat Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
E1A Binding Protein P300 (EP300) ELISA KitEP300 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Assay Time
- 4 - 5 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 6 months
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- p300 (EP300) (E1A Binding Protein P300 (EP300))
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Alternative Name
- p300
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Background
- Gene Aliases: E1A-associated protein p300, EP300, P300 transcription coactivator homolog
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Gene ID
- 2033
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UniProt
- Q09472
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Pathways
- p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
Target See all p300 (EP300) ELISA Kits
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