E2F1 ELISA Kit

Catalog No. ABIN8078344

Human and Mouse E2F1 ELISA Kit, Colorimetric assay for quantification of Human and Mouse E2F1.

Quick Overview for E2F1 ELISA Kit (ABIN8078344)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details E2F1 ELISA Kit

Purpose: E2F1 Colorimetric Cell-Based ELISA Kit for detecting E2F1 in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: Gene Aliases: E2F-1, PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, RBBP3, Retinoblastoma binding protein 3, Retinoblastoma-associated protein 1, Transcription factor E2F1

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy