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FOXO3 ELISA Kit

Rat FOXO3 ELISA Kit, Colorimetric assay for quantification of Rat FOXO3.
Catalog No. ABIN8079448
$1,069.15
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for FOXO3 ELISA Kit (ABIN8079448)

Target

See all FOXO3 ELISA Kits
FOXO3 (Forkhead Box O3 (FOXO3))

Reactivity

  • 25
  • 15
  • 9
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.312 ng/mL - 20 ng/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
  • Minimum Detection Limit

    0.312 ng/mL

    Purpose

    Rat Forkhead Box Protein O3 (Foxo3) ELISA Kit for detecting FOXO3 in tissue homogenates, cell lysates, cell culture supernates

    Analytical Method

    Quantitative

    Sensitivity

    0.121 ng/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all FOXO3 ELISA Kits

    FOXO3 (Forkhead Box O3 (FOXO3))

    Alternative Name

    Forkhead Box Protein O3

    Background

    Gene Aliases: FOX-O3, FOX2, FOXO3A, FKHRL1, FKHRL1P2, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1

    Gene ID

    294515

    UniProt

    D3ZBQ1

    Pathways

    Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis
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