GCK ELISA Kit
Quick Overview for GCK ELISA Kit (ABIN8079783)
Target
See all GCK ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.156 ng/mL
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Purpose
- Mouse Glucokinase (GCK) ELISA Kit for detecting GCK in serum, plasma, tissue homogenates
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Analytical Method
- Quantitative
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Sensitivity
- 0.054 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Glucokinase (Hexokinase 4) (GCK) ELISA Kit
GCK Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK Reactivity: Rat Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK Reactivity: Dog Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- GCK (Glucokinase (Hexokinase 4) (GCK))
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Alternative Name
- Glucokinase
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Background
- Gene Aliases: GK, GLK, HHF3, HK4, HKIV, HXKP, MODY2, Hexokinase-D, Hexokinase 4, Maturity Onset Diabetes Of The Young 2
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Gene ID
- 103988
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UniProt
- P52792
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Pathways
- MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
Target See all GCK ELISA Kits
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