IGF1 ELISA Kit

Catalog No. ABIN8081192

This Colorimetric ELISA kit is designed for the quantitative measurement of Dog IGF1.

Quick Overview for IGF1 ELISA Kit (ABIN8081192)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Dog

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details IGF1 ELISA Kit

Minimum Detection Limit: 78 pg/mL

Purpose: Dog Insulin Like Growth Factor 1 (IGF1) ELISA Kit for detecting IGF1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensitivity: 29 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: Insulin Like Growth Factor 1

Background: Gene Aliases: IGFI, IGF1A, IBP1, MGF, Somatomedin C, Mechano Growth Gactor

Gene ID: 610255

UniProt: P33712

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation