MME ELISA Kit
Quick Overview for MME ELISA Kit (ABIN8083454)
Target
See all MME ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 31.2 pg/mL
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Purpose
- Human Neprilysin (CD10) ELISA Kit for detecting CD10 in serum, plasma, cerebrospinal fluid
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Analytical Method
- Quantitative
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Sensitivity
- 12.5 pg/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Membrane Metallo-Endopeptidase (MME) ELISA Kit
MME Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME Reactivity: Rat Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME Reactivity: Rat Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME Reactivity: Mouse Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME Reactivity: Rat Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
SensoLyte® 520 Neprilysin Activity Assay KitAct, FRET Fluorometric
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- MME (Membrane Metallo-Endopeptidase (MME))
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Alternative Name
- Neprilysin
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Background
- Gene Aliases: NEP, MME, CALLA, SFE, Atriopeptidase, Enkephalinase, Skin fibroblast elastase, Common Acute Lymphoblastic Leukemia Antigen, Membrane Metallo-Endopeptidase
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Gene ID
- 4311
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UniProt
- P08473
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Pathways
- RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration
Target See all MME ELISA Kits
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