ERK2 ELISA Kit
Quick Overview for ERK2 ELISA Kit (ABIN8083671)
Target
See all ERK2 (MAPK1) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- p42 MAPK Colorimetric Cell-Based ELISA for detecting p42 MAPK in researchers plate their cell line of choice.
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Analytical Method
- Qualitative
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA Kit
MAPK1 Reactivity: Human Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA KitMAPK1 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA KitMAPK1 Reactivity: Rat Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA KitMAPK1 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
ERK Phosphorylation Assay KitBCA Reactivity: Human, Rat, Mouse
Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA KitMAPK1 Reactivity: Human Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA KitSmall Sample MAPK1 Reactivity: Human Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA KitMAPK1 Reactivity: Rat Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA KitMAPK1 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Assay Time
- 4 - 5 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 6 months
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- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
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Alternative Name
- p42 MAPK
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Background
- Gene Aliases: ERK-2, ERK2, ERT1, Extracellular signal- regulated kinase 2, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK, MAPK 2, MAPK1, MK01, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, P42-MAPK, PRKM1, kinase ERK2, p42-MAPK
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Gene ID
- 5594
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UniProt
- P28482
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Pathways
- MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
Target See all ERK2 (MAPK1) ELISA Kits
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