MEK1 ELISA Kit
Quick Overview for MEK1 ELISA Kit (ABIN8083702)
Target
See all MEK1 (MAP2K1) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- MEK1 Colorimetric Cell-Based ELISA Kit for detecting MEK1 in researchers plate their cell line of choice.
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Analytical Method
- Qualitative
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA Kit
MAP2K1 Reactivity: Human, Rat, Mouse pSer217 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 Reactivity: Human, Rat, Mouse pSer217 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Assay Time
- 4 - 5 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 6 months
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- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
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Alternative Name
- MEK1
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Background
- Gene Aliases: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1
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Gene ID
- 5604
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UniProt
- Q02750
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Pathways
- MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
Target See all MEK1 (MAP2K1) ELISA Kits
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