MAP2K7 ELISA Kit

Catalog No. ABIN8083724

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Mouse and Rat MAP2K7.

Quick Overview for MAP2K7 ELISA Kit (ABIN8083724)

Target: MAP2K7 (Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))

Binding Specificity: pThr275

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details MAP2K7 ELISA Kit

Purpose: Phospho-MAP2K7 (Thr275) Colorimetric Cell-Based ELISA Kit for detecting Phospho-MAP2K7 (Thr275) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for MAP2K7

Target: MAP2K7 (Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))

Alternative Name: MAP2K7

Background: Gene Aliases: c-Jun N-terminal kinase kinase 2, Dual specificity mitogen-activated protein kinase kinase 7, JNK activating kinase 2, JNK kinase 2, JNKK 2, JNKK2, kinase MKK7, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MP2K7, PRKMK7

Gene ID: 5609

UniProt: O14733

Pathways: MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling