NF-kB p65 ELISA Kit
Quick Overview for NF-kB p65 ELISA Kit (ABIN8084544)
Target
See all NF-kB p65 (NFkBP65) ELISA KitsBinding Specificity
Reactivity
Detection Method
Method Type
Application
Sample Type
-
-
Purpose
- Phospho-NF-κB p65 (Ser529) Colorimetric Cell-Based ELISA Kit for detecting Phospho-NF-κB p65 (Ser529) in researchers plate their cell line of choice.
-
Analytical Method
- Qualitative
-
Components
-
- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
-
Material not included
-
- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
-
-
Nuclear Factor-kB p65 (NFkBP65) ELISA Kit
NFkBP65 Reactivity: Human Colorimetric DNA-Binding ELISA
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Rat Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Human Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Mouse Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Rat Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Comment
-
This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
-
Assay Time
- 4 - 5 h
-
Plate
- Pre-coated
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- 4°C for one month, Long term: see manual.
-
Expiry Date
- 6 months
-
-
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
-
Alternative Name
- NF-kappaB p65
-
Background
- Gene Aliases: NFKB3, nuclear factor NF-kappa-B p65 subunit, p65, RELA, TF65, transcription factor p65
-
Gene ID
- 5970
-
UniProt
- Q04206
-
Pathways
- NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
Target See all NF-kB p65 (NFkBP65) ELISA Kits
-
