PRKAA1 ELISA Kit
Quick Overview for PRKAA1 ELISA Kit (ABIN8085885)
Target
See all PRKAA1 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.156 ng/mL
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Purpose
- Human Protein Kinase, AMP Activated Alpha 1 (AMPK Alpha 1) ELISA Kit for detecting AMPK Alpha 1 in tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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Sensitivity
- 0.059 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA Kit
PRKAA1 Reactivity: Human, Mouse, Rat pSer487 Colorimetric Sandwich ELISA Cell Culture Lysate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 Reactivity: Human, Mouse, Rat pSer487 Colorimetric Sandwich ELISA Cell Culture Lysate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 Reactivity: Human Colorimetric Competition ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 Reactivity: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 Reactivity: Human Colorimetric Competition ELISA 78.125 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))
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Alternative Name
- Protein Kinase, AMP Activated Alpha 1
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Background
- Gene Aliases: PRKAa1, AMPK, AMPKa1, PKA, ACACA kinase, 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase
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Gene ID
- 5562
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UniProt
- Q13131
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Pathways
- AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect
Target See all PRKAA1 ELISA Kits
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