SHP1 ELISA Kit

Catalog No. ABIN8085971

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Mouse and Rat SHP1.

Quick Overview for SHP1 ELISA Kit (ABIN8085971)

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Binding Specificity: pTyr536

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details SHP1 ELISA Kit

Purpose: Phospho-SHP-1 (Tyr536) Colorimetric Cell-Based ELISA Kit for detecting Phospho-SHP-1 (Tyr536) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for SHP1

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Alternative Name: SHP-1

Background: Gene Aliases: 70Z-SHP, HCP, HCPH, Hematopoietic cell protein-tyrosine phosphatase, PTN6, PTP-1C, PTP1C, PTPN6, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, Protein-tyrosine phosphatase, non-receptor type 6, SH-PTP1

Gene ID: 5777

UniProt: P29350

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling