SKP1 ELISA Kit

Catalog No. ABIN8086474

Human SKP1 ELISA Kit, Colorimetric assay for quantification of Human SKP1.

Quick Overview for SKP1 ELISA Kit (ABIN8086474)

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details SKP1 ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: Human S-Phase Kinase Associated Protein 1 (SKP1) ELISA Kit for detecting SKP1 in tissue homogenates, cell lysates

Analytical Method: Quantitative

Sensitivity: 0.062 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: S-Phase Kinase Associated Protein 1

Background: Gene Aliases: SKP1A, EMC19, OCP-II, OCP2, TCEB1L, p19A, Cyclin-A/CDK2-associated p19, RNA polymerase II elongation factor-like protein, Transcription elongation factor B polypeptide 1-like

Gene ID: 6500

UniProt: P63208

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases