SKP1 ELISA Kit

Catalog No. ABIN8086475

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Mouse and Rat SKP1.

Quick Overview for SKP1 ELISA Kit (ABIN8086475)

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details SKP1 ELISA Kit

Purpose: SKP1A/p19 Colorimetric Cell-Based ELISA Kit for detecting SKP1A/p19 in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: SKP1A/p19

Background: Gene Aliases: EMC19, OCP-2, OCP-II, OCP2, RNA polymerase II elongation factor-like, RNA polymerase II elongation factor-like OCP2, S-phase kinase-associated 1A, S-phase kinase-associated 1A (p19A) SIII, SKP1, TCEB1L, cyclin A/CDK2-associated p19, organ of Corti 2, p19A, p19skp1, transcription elongation factor B, transcription elongation factor B (SIII) polypeptide 1-like

Gene ID: 6500

UniProt: P63208

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases