TLR4 ELISA Kit
Quick Overview for TLR4 ELISA Kit (ABIN8088070)
Target
See all TLR4 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.625 ng/mL
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Purpose
- Bovine Toll Like Receptor 4 (TLR4) ELISA Kit for detecting TLR4 in serum, plasma, tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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Sensitivity
- 0.281 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Toll-Like Receptor 4 (TLR4) ELISA Kit
TLR4 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Toll-Like Receptor 4 (TLR4) ELISA KitTLR4 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Toll-Like Receptor 4 (TLR4) ELISA KitTLR4 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Toll-Like Receptor 4 (TLR4) ELISA KitTLR4 Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Toll-Like Receptor 4 (TLR4) ELISA KitTLR4 Reactivity: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Toll-Like Receptor 4 (TLR4) ELISA KitTLR4 Reactivity: Mouse Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Toll-Like Receptor 4 (TLR4) ELISA KitTLR4 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Toll-Like Receptor 4 (TLR4) ELISA KitTLR4 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Toll-Like Receptor 4 (TLR4) ELISA KitTLR4 Reactivity: Cow Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- TLR4 (Toll-Like Receptor 4 (TLR4))
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Alternative Name
- Toll Like Receptor 4
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Background
- Gene Aliases: CD284, TOLL, HToll
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Gene ID
- 281536
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UniProt
- Q9GL65
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Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins
Target See all TLR4 ELISA Kits
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