Angiotensin II ELISA Kit

Catalog No. ABIN8089438

Cow Angiotensin II ELISA Kit, Colorimetric assay for quantification of Cow Angiotensin II.

Quick Overview for Angiotensin II ELISA Kit (ABIN8089438)

Target: Angiotensin II (Ang II)

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 1.563 pg/mL - 100 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Product Details Angiotensin II ELISA Kit

Minimum Detection Limit: 1.563 pg/mL

Purpose: Bovine Angiotensin II (Angiotensin II) ELISA Kit

Analytical Method: Quantitative

Specificity: Specifically binds with ANGII , no obvious cross reaction with other analogues.

Sensitivity: 0.938 pg/mL

Components:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• HRP-Antigen(Concentrated
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Material not included:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Competitive ELISA, Coated with Antibody

Assay Time: 1 h

Plate: Pre-coated

Protocol: Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 100 µL standard/sample/HRP-labeled antigen working solution into each well. Seal the plate and statically incubate for 45 minutes at 37 °C. (Equilibrate the whole bottle of TMB for 30 min at 37 °C.) Washing: Wash the plate five times and immerse for 1 min each time. Step 3: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid direct light exposure to antibody solutions

Storage: 4 °C

Storage Comment: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Expiry Date: 12 months

Target Details for Angiotensin II

Target: Angiotensin II (Ang II)

Alternative Name: ANG II

Background: ANG II, Angiotensin II

UniProt: P01017

Pathways: JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity