NF-kB p65 ELISA Kit

Catalog No. ABIN8126254

The Human NF-kB p65 ELISA Kit (ABIN8126254) is a Colorimetric ELISA Kit designed to quantify Human NF-kB p65.

Quick Overview for NF-kB p65 ELISA Kit (ABIN8126254)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.313 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details NF-kB p65 ELISA Kit

Minimum Detection Limit: 0.313 ng/mL

Purpose: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensitivity: 0.188 ng/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human NF-kB p65 concentrations in serum, plasma. It also detects cytoplasmic, nuclear and total NF-kB p65 from cells. Detection Range: 0.313 - 20 ng/mL Sensitivity: < 0.188 ng/mL

Application Details

Plate: Pre-coated

Protocol: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human p65 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human p65 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human p65 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human p65 can be calculated using the standard curve. Kit Components

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the manual

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: NF-kB p65 (Nuclear Factor Kappa B p65)

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins