Insulin ELISA Kit

Catalog No. ABIN997069

Human Insulin ELISA Kit, Colorimetric assay for quantification of Human Insulin.

Quick Overview for Insulin ELISA Kit (ABIN997069)

Target: Insulin (INS)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0-200 μIU/mL

Application: ELISA

Sample Type: Serum

Product Details Insulin ELISA Kit

Minimum Detection Limit: 0 μIU/mL

Purpose: Insulin Microplate Elisa test is intended to be used for the quantitative determination of insulin levels in human serum.

Analytical Method: Quantitative

Sensitivity: 1.5 μIU/mL

Application Details

Sample Volume: 25 μL

Assay Time: < 1 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12-14 months

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Insulin is the principal hormone responsible for glucose metabolism. It is synthesized in the cells of the islets of Langerhans as the precuror, proinsulin, which is processed to form C-peptide and insulin and both are secreted in equimolar amounts into the portal circulation. The mature insulin molecule comprises two polypeptide chairs, the A chain (21 amino acids) and the B chain (30 amino acids), which are linked by two inter-chain disulphide bridges. There is, in addition, a single intra-chain disulphide bridge in the A chain. The sequence of insulin is highly conserved in mammalian species, and is homologous with the insulin-like growth factors IGF-I and IGF-II. Secretion of insulin is mainly controlled by plasma glucose concentration and the hormones have a number of important metabolic actions. Its principal function is to control the uptake and utilization of glucose in peripheral tissues via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by the hyperglycaemic hormones including glucagons, epinephrine(adrenaline), growth hormone and cortisol. Insulin concentrations are severely reduced in insulin-dependent diabetes (DDM) and some other conditions such as hypopituitarism. Insulin concentrations may be raised in non-insulin- dependant diabetes (NIDDM), obesity, insulinoma and some endocrine dysfunctions such as Cushing?s Syndrome and Acromegaly.

The main clinical utility measurement is in the investigation of hypoglycaemia. Insulin assay have been used in the following applications:
1. To assess the residual cell function, especially in newly diagnosed cases of IDDM.
2. As an aid to the discrimination between IDDM and NIDDM.
3. The diagnosis of insulinoma.
4. In the investigation of the pathophysiology of diabetes mellitus.
Insulin assays are the essentials in various dynamic tests, such as oral of intravenous glucose tolerance tests (OGTT and IVGTT), to determine the insulin response of the pancreas and the degree of insulin resistance. In many applications, insulin measurements may be complicated by cross-reactivity with partially degraded insulin, proinsulin and split forms of proinsulin. Immune complexes of these molecules are essentially problematic in patients who have developed anti-insulin antibodies through animal insulin administration.

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation