AF5Q31 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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- Target See all AFF4 products
- AFF4 (AF4/FMR2 Family, Member 4 (AFF4))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
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AF5Q31 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-AF5Q31 full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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AFF4 293T Cell Transient Overexpression Lysate(Denatured)
AFF4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
AFF4 293T Cell Transient Overexpression Lysate(Denatured)AFF4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
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Product Quality tested by: Transient overexpression cell lysate was tested with Anti-AF5Q31 antibody by Western Blots.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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- Target
- AFF4 (AF4/FMR2 Family, Member 4 (AFF4))
- Alternative Name
- AFF4 (AFF4 Products)
- Synonyms
- AFF4 Lysate, RA_m002_jsmFBA6Br Lysate, AF5Q31 Lysate, MCEF Lysate, Alf4 Lysate, Laf4l Lysate, AF4/FMR2 family member 4 L homeolog Lysate, AF4/FMR2 family member 4 Lysate, AF4/FMR2 family, member 4 Lysate, aff4.L Lysate, AFF4 Lysate, aff4 Lysate, Aff4 Lysate
- Background
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Full Gene Name: AF4/FMR2 family, member 4
Synonyms: AF5Q31,MCEF,MGC75036 - Gene ID
- 27125
- NCBI Accession
- NP_055238, NM_014423
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