Rat brain tissue lysate (non-denatured)
(1 reference)-
- Protein Species
- Rat
- Species of Lysate
- Rat
- Application
- Immunoprecipitation (IP), Western Blotting (WB)
- Purpose
- Whole tissue lysate
- Specificity
- Non-denatured
- Characteristics
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Rat brain tissue lysate (non-denatured)
Used Tissue: Brain - Lysate Type
- Tissue Lysate
- Lysate Fraction
- Whole Tissue Lysate
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BIRC3 293T Cell Transient Overexpression Lysate(Denatured)
BIRC3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
APLP2 293T Cell Transient Overexpression Lysate(Denatured)APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
BAG1 293T Cell Transient Overexpression Lysate(Denatured)BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
VNN1 293T Cell Transient Overexpression Lysate(Denatured)VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
Hep2 Whole Cell LysateWB Whole Cell Lysate Cell Lysate HepG2 Cells Reactivity: Human
NIH/3T3 Whole Cell Lysate (PDGF Stimulated)WB Whole Cell Lysate Cell Lysate 3T3 Cells PDGF Stimulated Reactivity: Mouse
DYKDDDDK Positive Control LysatePC, WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Tag
A431 Whole Cell Lysate (EGF Stimulated)WB Whole Cell Lysate Cell Lysate A431 Cells EGF treated Reactivity: Human
CD177 (human): 293T LysateCD177 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells
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- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
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Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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: "Improved coomassie blue dye-based fast staining protocol for proteins separated by SDS-PAGE." in: PLoS ONE, Vol. 8, Issue 11, pp. e81696, (2013) (PubMed).
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: "Improved coomassie blue dye-based fast staining protocol for proteins separated by SDS-PAGE." in: PLoS ONE, Vol. 8, Issue 11, pp. e81696, (2013) (PubMed).
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- Background
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Full Gene Name:
Synonyms:
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