CYB5 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
-
- Target See all CYB5A products
- CYB5A (Cytochrome B5 Type A (Microsomal) (CYB5A))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Immunoprecipitation (IP), Western Blotting (WB)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
-
CYB5 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-CYB5 full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
-
- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
-
Product Quality tested by: Transient overexpression cell lysate was tested with Anti-CYB5 antibody by Western Blots.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
-
- Target
- CYB5A (Cytochrome B5 Type A (Microsomal) (CYB5A))
- Alternative Name
- CYB5A (CYB5A Products)
- Synonyms
- CYB5 Lysate, MCB5 Lysate, Cyb5 Lysate, cyb5 Lysate, zgc:64123 Lysate, wu:fb44a11 Lysate, MGC80327 Lysate, 0610009N12Rik Lysate, Cyb5a Lysate, CYB5A Lysate, cytochrome b5 type A Lysate, cytochrome b5 type A (microsomal) Lysate, cytochrome b5 type A (microsomal) L homeolog Lysate, cytochrome b5 Lysate, CYB5A Lysate, Cyb5a Lysate, cyb5a Lysate, cyb5a.L Lysate, LOC100328915 Lysate, LOC100735904 Lysate
- Background
-
Full Gene Name: cytochrome b5 type A (microsomal)
Synonyms: CYB5,MCB5 - Gene ID
- 1528
-