COG7 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
COG7
IP, WB
Whole Cell Lysate
Overexpression Lysate
HEK 293T Cells
Reactivity: Human
-
- Target See all COG7 products
- COG7 (Component of Oligomeric Golgi Complex 7 (COG7))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Immunoprecipitation (IP), Western Blotting (WB)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
-
COG7 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-COG7 full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
-
Product Quality tested by: Transient overexpression cell lysate was tested with Anti-COG7 antibody by Western Blots.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
-
- Target
- COG7 (Component of Oligomeric Golgi Complex 7 (COG7))
- Alternative Name
- COG7 (COG7 Products)
- Synonyms
- CG31040 Lysate, CG7880 Lysate, Dmel\\CG31040 Lysate, MGC80865 Lysate, COG7 Lysate, MGC107838 Lysate, zgc:152912 Lysate, zgc:153470 Lysate, CDG2E Lysate, 5630400E24Rik Lysate, Gm167 Lysate, CG31040 gene product from transcript CG31040-RA Lysate, component of oligomeric golgi complex 7 Lysate, component of oligomeric golgi complex 7 L homeolog Lysate, Cog7 Lysate, COG7 Lysate, cog7.L Lysate, cog7 Lysate
- Background
-
Full Gene Name: component of oligomeric golgi complex 7
Synonyms: CDG2E - Gene ID
- 91949
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