EDN1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
EDN1
WB, IP
Whole Cell Lysate
Overexpression Lysate
HEK 293T Cells
Reactivity: Human
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- Target See all Endothelin 1 (EDN1) products
- Endothelin 1 (EDN1)
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
-
EDN1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-EDN1 full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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EDN1 293T Cell Transient Overexpression Lysate(Denatured)
EDN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
-
Product Quality tested by: Transient overexpression cell lysate was tested with Anti-EDN1 antibody by Western Blots.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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- Target
- Endothelin 1 (EDN1)
- Alternative Name
- EDN1 (EDN1 Products)
- Synonyms
- et1 Lysate, et-1 Lysate, Edn1 Lysate, EDN Lysate, ET-1 Lysate, ET1 Lysate, PPET1 Lysate, HDLCQ7 Lysate, preproET Lysate, Et1 Lysate, fb14d01 Lysate, wu:fb14d01 Lysate, PPET-1 Lysate, EDN1 Lysate, edn1 Lysate, edn1-a Lysate, edn1-b Lysate, endothelin 1 Lysate, endothelin 1 S homeolog Lysate, endothelin 1 L homeolog Lysate, edn1 Lysate, EDN1 Lysate, Edn1 Lysate, edn1.S Lysate, edn1.L Lysate
- Background
-
Full Gene Name: endothelin 1
Synonyms: ET1,HDLCQ7 - Gene ID
- 1906
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