EEF1G HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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- Target See all EEF1G products
- EEF1G (Eukaryotic Translation Elongation Factor 1 gamma (EEF1G))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
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EEF1G HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-EEF1G full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
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Product Quality tested by: Transient overexpression cell lysate was tested with Anti-EEF1G antibody by Western Blots.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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- Target
- EEF1G (Eukaryotic Translation Elongation Factor 1 gamma (EEF1G))
- Alternative Name
- EEF1G (EEF1G Products)
- Synonyms
- EF1G Lysate, GIG35 Lysate, eEF-1 gamma Lysate, db03h10 Lysate, fk76a09 Lysate, mg:db03h10 Lysate, wu:fk76a09 Lysate, 2610301D06Rik Lysate, AA407312 Lysate, eef1g Lysate, ef1g Lysate, gig35 Lysate, eukaryotic translation elongation factor 1 gamma Lysate, eukaryotic translation elongation factor 1 gamma L homeolog Lysate, eukaryotic translation elongation factor 1 gamma S homeolog Lysate, EEF1G Lysate, eef1g Lysate, Eef1g Lysate, eef1g.L Lysate, eef1g.S Lysate, EHI_119540 Lysate, CMU_014590 Lysate
- Background
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Full Gene Name: eukaryotic translation elongation factor 1 gamma
Synonyms: EF1G,GIG35 - Gene ID
- 1937
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