FYN HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
FYN
WB, IP
Whole Cell Lysate
Overexpression Lysate
HEK 293T Cells
Reactivity: Human
-
- Target See all FYN products
- FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
-
FYN HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-FYN full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
-
FYN 293T Cell Transient Overexpression Lysate(Denatured)
FYN WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
-
- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
-
Product Quality tested by: Transient overexpression cell lysate was tested with Anti-FYN antibody by Western Blots.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
-
- Target
- FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))
- Alternative Name
- FYN (FYN Products)
- Synonyms
- im:6908851 Lysate, si:dkey-119m7.5 Lysate, zgc:109996 Lysate, c-fyn Lysate, cfyn-a Lysate, SLK Lysate, SYN Lysate, p59-FYN Lysate, PKC Lysate, fyn Lysate, zgc:86720 Lysate, AI448320 Lysate, AW552119 Lysate, FYN proto-oncogene, Src family tyrosine kinase Lysate, FYN proto-oncogene, Src family tyrosine kinase S homeolog Lysate, FYN proto-oncogene, Src family tyrosine kinase b Lysate, FYN proto-oncogene, Src family tyrosine kinase L homeolog Lysate, FYN proto-oncogene, Src family tyrosine kinase a Lysate, Fyn proto-oncogene Lysate, FYN Lysate, fyn.S Lysate, fynb Lysate, fyn.L Lysate, Fyn Lysate, fyna Lysate
- Background
-
Full Gene Name: FYN oncogene related to SRC, FGR, YES
Synonyms: MGC45350,SLK,SYN - Gene ID
- 2534
-
You are here:
