SFRP4 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
SFRP4
IP, WB
Whole Cell Lysate
Overexpression Lysate
HEK 293T Cells
Reactivity: Human
-
- Target See all SFRP4 products
- SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Immunoprecipitation (IP), Western Blotting (WB)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
-
SFRP4 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-SFRP4 full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
-
- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
-
Product Quality tested by: Transient overexpression cell lysate was tested with Anti-SFRP4 antibody by Western Blots.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
-
- Target
- SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))
- Alternative Name
- SFRP4 (SFRP4 Products)
- Synonyms
- SFRP4 Lysate, FRP-4 Lysate, FRPHE Lysate, sFRP-4 Lysate, secreted frizzled related protein 4 Lysate, secreted frizzled-related protein 4 Lysate, SFRP4 Lysate, Sfrp4 Lysate
- Background
-
Full Gene Name: secreted frizzled-related protein 4
Synonyms: FRP-4,FRPHE,MGC26498 - Gene ID
- 6424
- NCBI Accession
- NP_003005, NM_003014
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