AKR1C2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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- Target See all AKR1C2 products
- AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
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AKR1C2 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-AKR1C2 full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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AKR1C2 293T Cell Transient Overexpression Lysate(Denatured)
AKR1C2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
AKR1C2 293T Cell Transient Overexpression Lysate(Denatured)AKR1C2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
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Product Quality tested by: Transient overexpression cell lysate was tested with Anti-AKR1C2 antibody by Western Blots.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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- Target
- AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))
- Alternative Name
- AKR1C2 (AKR1C2 Products)
- Synonyms
- AKR1C-pseudo Lysate, BABP Lysate, DD Lysate, DD2 Lysate, DDH2 Lysate, HAKRD Lysate, HBAB Lysate, MCDR2 Lysate, SRXY8 Lysate, Akr1c21 Lysate, aldo-keto reductase family 1 member C2 Lysate, aldo-keto reductase family 1, member C2 Lysate, AKR1C2 Lysate, Akr1c2 Lysate
- Background
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Full Gene Name: aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2, bile acid binding protein, 3-alpha hydroxysteroid dehydrogenase, type III)
Synonyms: AKR1C-pseudo,BABP,DD,DD2,DDH2,HAKRD,HBAB,MCDR2 - Gene ID
- 1646
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