Lung Membrane Lysate
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- Species of Lysate
- Human
- Application
- Western Blotting (WB)
- Characteristics
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Human lung tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human lung tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The membrane protein is provided in a buffer including HEPES ( pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated lung tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated lung tissue membrane protein is then Western analyzed by either GAPDH or beta-actin antibody to confirm there is no signal or very weak signal.
Source: Human Lung tissue
Diagnosis: Normal
Tissue or Cell type: Lung - Lysate Type
- Tissue Lysate
- Lysate Fraction
- Membrane Fraction
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- Application Notes
- Lung membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
- Handling Advice
- Repeated freezing and thawing is not recommended.
- Storage
- 4 °C/-80 °C
- Storage Comment
- Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
- Expiry Date
- 6 months
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