anti-Human HSPB1 antibody for Immunofluorescence

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Epitope
Center
86
73
56
45
22
16
13
13
10
10
10
9
6
6
5
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
833
277
272
96
85
71
68
66
36
30
29
26
18
11
11
9
6
3
2
2
2
2
1
1
1
Host
Rabbit
508
343
2
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
33
23
22
19
13
13
13
11
11
10
10
10
10
10
9
9
9
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
725
337
274
266
231
213
166
157
59
40
17
14
11
8
6
4
2
2
1
1
1
Options
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Catalog No. ABIN2854951
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.714748 ABIN410085 ICC IF IHC (p) IP IHC WB Mouse IgG1 kappa Log in to see 8A7 0
11.714748 ABIN4319980 ICC IF IHC IHC (p) WB Rabbit AA 80-150, Internal Region Log in to see Polyclonal 2
11.714748 ABIN267333 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
11.714748 ABIN1873103 IF WB Rabbit IgG Log in to see Polyclonal 0
11.714748 ABIN1870247 IF IHC WB Rabbit IgG pSer15 Log in to see Polyclonal 0
11.714748 ABIN1870249 IF IHC WB Rabbit IgG pSer78 Log in to see Polyclonal 0
11.714748 ABIN4320008 CyTOF FACS ICC IF WB Mouse IgG1 Log in to see 6H11 0
11.714748 ABIN1870246 IF IHC WB Rabbit IgG pSer82 Log in to see Polyclonal 0
11.714748 ABIN516740 IF IP PLA ELISA WB Mouse IgG2a kappa AA 96-205, partial Log in to see 3G3 0
11.714748 ABIN1996519 FACS IF IF/ICC Rabbit IgG AA 1-205 Log in to see 155 0
11.714748 ABIN4319976 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 5D7 0
11.714748 ABIN4320010 ICC IF WB Chicken Log in to see Polyclonal 0
11.714748 ABIN2649210 FACS IF IF/ICC Mouse IgG1 AA 1-205 Log in to see 09 0
11.714748 ABIN1107614 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5D7 2
11.714748 ABIN2425005 IF IHC WB Rabbit IgG pSer78 Log in to see Polyclonal 0
11.714748 ABIN2424911 IF IHC WB Rabbit IgG pSer15 Log in to see Polyclonal 0
11.714748 ABIN3019755 IF IHC WB Rabbit pSer15 Log in to see Polyclonal 0
11.714748 ABIN3019893 IF IHC WB Rabbit pSer78 Log in to see Polyclonal 0
1 ABIN361756 FACS ICC IF IHC IP WB Mouse IgG1 kappa Log in to see 8A7 9
1 ABIN361649 ICC IF IP IHC ELISA WB Mouse IgG2b kappa Log in to see 5D12-A12 7

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope Center
(86), (73), (56), (45), (22), (16), (13), (13), (10), (10), (10), (9), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(833), (277), (272), (96), (85), (71), (68), (66), (36), (30), (29), (26), (18), (11), (11), (9), (6), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(508), (343), (2)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(34), (33), (23), (22), (19), (13), (13), (13), (11), (11), (10), (10), (10), (10), (10), (9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(725), (337), (274), (266), (231), (213), (166), (157), (59), (40), (17), (14), (11), (8), (6), (4), (2), (2), (1), (1), (1)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to HSP27 (heat shock 27 kDa protein 1)
HSP27 antibody
Purification Affinity purified by Protein A.
Immunogen Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
Isotype IgG

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Cellular Localization: Cytoplasm , Nucleus , Spindle
Molecular Weight 23 kDa
Gene ID 3315
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Suggested dilution Reference Flow cytometry/FACS Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACSAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:10000*
Comment

Positive Control: A431 , HeLa , mouse liver , *HT29

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Liquid
Concentration 3.23 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody (ABIN2854951) WB Image Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE antibody dil...
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody (ABIN2854951) IHC-P Image HSP27 antibody detects HSPB1 protein at cytosol on U87 xenograft by immun...
Immunoprecipitation (IP) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody (ABIN2854951) IP Image Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 μ...
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody (ABIN2854951) WB Image HSP27 antibody detects HSP27 protein by western blot analysis. A. 30 μg A431...
Immunofluorescence (IF) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody (ABIN2854951) ICC/IF Image HSP27 antibody detects HSP27 protein at cytoplasm and cytoskeleton by im...