anti-Sheep (Ovine) HSPB1 antibody for Western Blotting

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
968
373
345
161
133
101
85
67
67
61
46
41
36
29
13
11
7
4
3
2
2
2
1
1
1
1
Host
Mouse
558
428
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is conjugated to Atto 488
41
39
27
27
22
18
14
12
12
11
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
8
7
6
6
6
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
841
428
290
285
262
238
214
210
75
59
42
42
40
18
14
6
5
4
2
2
1
1
1
Options
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Available images

Immunoprecipitation (IP) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2481412)
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2481412)
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2481412)
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2481412)
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2481412)
Catalog No. ABIN2481412
$ 456.30
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.392223 ABIN2481415 FACS ICC IF IHC IP WB Atto 633 Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481419 FACS ICC IF IHC IP WB Alkaline Phosphatase (AP) Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481420 FACS ICC IF IHC IP WB APC Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481411 FACS ICC IF IHC IP WB Atto 390 Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481413 FACS ICC IF IHC IP WB Atto 565 Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481414 FACS ICC IF IHC IP WB Atto 594 Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481417 FACS ICC IF IHC IP WB Atto 680 Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481416 FACS ICC IF IHC IP WB Atto 655 Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481418 FACS ICC IF IHC IP WB Atto 700 Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481421 FACS ICC IF IHC IP WB Biotin Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481422 FACS ICC IF IHC IP WB FITC Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481423 FACS ICC IF IHC IP WB HRP Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481424 FACS ICC IF IHC IP WB PE-Atto 594 Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481426 FACS ICC IF IHC IP WB PE Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481427 FACS ICC IF IHC IP WB Streptavidin Mouse IgG1 kappa Log in to see 8A7 0
28.392223 ABIN2481425 FACS ICC IF IHC IP WB PerCP Mouse IgG1 kappa Log in to see 8A7 0
19.392223 ABIN361756 FACS ICC IF IHC IP WB Mouse IgG1 kappa Log in to see 8A7 9
10 ABIN3026467 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see G3-1 0
7 ABIN6164157 FACS IF IHC (f) ISt WB APC Mouse IgG1, kappa Log in to see G3-1 0
7 ABIN6164164 FACS IF IHC (f) ISt WB PerCP Mouse IgG1, kappa Log in to see G3-1 0

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(968), (373), (345), (161), (133), (101), (85), (67), (67), (61), (46), (41), (36), (29), (13), (11), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(558), (428), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is conjugated to Atto 488
(41), (39), (27), (27), (22), (18), (14), (12), (12), (11), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(841), (428), (290), (285), (262), (238), (214), (210), (75), (59), (42), (42), (40), (18), (14), (6), (5), (4), (2), (2), (1), (1), (1)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Specificity Detects ~25 kDa or ~27 kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
Purification Protein G Purified
Immunogen HSP27 peptide
Clone 8A7
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background HSP25 is the mouse homologue of the human HSP27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa(1). The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, HSP27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermo-tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (8). For more information visit our HSP27 Scientific Resource Guide at http://www.HSP27.com.
Gene ID 3315
NCBI Accession NP_001532
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes
  • WB (1:5000)
  • ICC/IF (1:200)
  • IHC (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2481412) Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 ....
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2481412) Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 ....
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2481412) Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 ....
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2481412) Western Blot analysis of Rat Lung tissue lysates showing detection of Hsp27 protein u...
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2481412) Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 ....