anti-Sheep (Ovine) HSPB1 antibody for Western Blotting

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
1009
383
357
162
134
103
88
68
68
61
48
43
36
30
13
11
7
4
3
2
2
2
1
1
1
1
Host
Mouse
576
453
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
42
40
29
28
24
18
16
13
13
12
10
10
10
10
10
9
9
9
8
8
8
8
8
8
8
8
8
6
6
6
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
883
457
315
298
289
239
217
215
75
59
42
42
40
18
14
6
5
4
2
2
1
1
1
Options
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Pubmed
9 references available

Available images

image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN361756)
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN361756)
Catalog No. ABIN361756
$ 334.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0797327 ABIN2481417 FACS ICC IF IHC IP WB Atto 680 Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481427 FACS ICC IF IHC IP WB Streptavidin Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481414 FACS ICC IF IHC IP WB Atto 594 Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481412 FACS ICC IF IHC IP WB Atto 488 Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481420 FACS ICC IF IHC IP WB APC Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481423 FACS ICC IF IHC IP WB HRP Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481411 FACS ICC IF IHC IP WB Atto 390 Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481413 FACS ICC IF IHC IP WB Atto 565 Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481415 FACS ICC IF IHC IP WB Atto 633 Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481416 FACS ICC IF IHC IP WB Atto 655 Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481418 FACS ICC IF IHC IP WB Atto 700 Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481419 FACS ICC IF IHC IP WB Alkaline Phosphatase (AP) Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481422 FACS ICC IF IHC IP WB FITC Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481421 FACS ICC IF IHC IP WB Biotin Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481424 FACS ICC IF IHC IP WB PE-Atto 594 Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481426 FACS ICC IF IHC IP WB PE Mouse IgG1 kappa Log in to see 8A7 0
3.0797327 ABIN2481425 FACS ICC IF IHC IP WB PerCP Mouse IgG1 kappa Log in to see 8A7 0
1 ABIN6164131 FACS IF IHC (f) ISt WB CF®405S Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164141 FACS IF IHC (f) ISt WB CF®555 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164147 FACS IF IHC (f) ISt WB CF®770 Mouse IgG1, kappa Log in to see G3-1 0

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(1009), (383), (357), (162), (134), (103), (88), (68), (68), (61), (48), (43), (36), (30), (13), (11), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(576), (453), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(42), (40), (29), (28), (24), (18), (16), (13), (13), (12), (10), (10), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(883), (457), (315), (298), (289), (239), (217), (215), (75), (59), (42), (42), (40), (18), (14), (6), (5), (4), (2), (2), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN361756) Images
Specificity Detects ~25 kDa or ~27 kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
Purification Protein G Purified
Immunogen HSP27 peptide
Clone 8A7
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 antibody (ABIN361756) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background HSP25 is the mouse homologue of the human HSP27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa(1). The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, HSP27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermo-tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (8). For more information visit our HSP27 Scientific Resource Guide at http://www.HSP27.com.
Gene ID 3315
NCBI Accession NP_001532
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 antibody (ABIN361756) Images back to top
Application Notes
  • WB (1:5000)
  • ICC/IF (1:200)
  • IHC (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 antibody (ABIN361756) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-HSPB1 antibody (ABIN361756)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Background publications

Arrigo: "In search of the molecular mechanism by which small stress proteins counteract apoptosis during cellular differentiation." in: Journal of cellular biochemistry, Vol. 94, Issue 2, pp. 241-6, 2005

Van Montfort, Slingsby, Vierling: "Structure and function of the small heat shock protein/alpha-crystallin family of molecular chaperones." in: Advances in protein chemistry, Vol. 59, pp. 105-56, 2002

Jia, Ransom, Shibanuma, Liu, Welsh, Smoyer: "Identification and characterization of hic-5/ARA55 as an hsp27 binding protein." in: The Journal of biological chemistry, Vol. 276, Issue 43, pp. 39911-8, 2001

Sarto, Binz, Mocarelli: "Heat shock proteins in human cancer." in: Electrophoresis, Vol. 21, Issue 6, pp. 1218-26, 2000

Kim, Kim, Kim: "Crystal structure of a small heat-shock protein." in: Nature, Vol. 394, Issue 6693, pp. 595-9, 1998

Ehrnsperger, Gräber, Gaestel, Buchner: "Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation." in: The EMBO journal, Vol. 16, Issue 2, pp. 221-9, 1997

Welsh, Wu, Parvinen, Gilmont: "Variation in expression of hsp27 messenger ribonucleic acid during the cycle of the seminiferous epithelium and co-localization of hsp27 and microfilaments in Sertoli cells of the rat." in: Biology of reproduction, Vol. 55, Issue 1, pp. 141-51, 1997

Ciocca, Oesterreich, Chamness, McGuire, Fuqua: "Biological and clinical implications of heat shock protein 27,000 (Hsp27): a review." in: Journal of the National Cancer Institute, Vol. 85, Issue 19, pp. 1558-70, 1993

Welch: "Phorbol ester, calcium ionophore, or serum added to quiescent rat embryo fibroblast cells all result in the elevated phosphorylation of two 28,000-dalton mammalian stress proteins." in: The Journal of biological chemistry, Vol. 260, Issue 5, pp. 3058-62, 1985

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN361756) back to top
Supplier Images
image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN361756) Hsp25 Hsp27 (8A7), somites of a rat embryo Courtesy of Mike Welsh, UMich .
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN361756) SMC 114 Rat LungTissue 10ug Hsp25 WB 1 in 1000.