anti-Sheep (Ovine) HSPB1 antibody for Western Blotting
Recommended HSPB1 Antibody (supplied by: Log in to see )
- Antigen
-
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
show synonyms for this antigen- CMT2F
- HMN2B
- HS.76067
- HSP27
- HSP28
- Hsp25
- SRP27
- 27kDa
- Hsp27
- cb153
- cb660
- hsp1
- hsp25
- hsp27
- id:ibd2821
- sb:cb660
- zgc:103437
- HSPB1
- cmt2f
- hs.76067
- hsp28
- heat shock protein family B (small) member 1
- heat shock protein 1
- heat shock protein, alpha-crystallin-related, 1
- heat shock protein family B (small) member 1 L homeolog
- HSPB1
- Hspb1
- hspb1
- hspb1.L
- Reactivity
-
Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
Alternatives - Host
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Mouse
Alternatives - Clonality
- Conjugate
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This HSPB1 antibody is un-conjugated
Alternatives - Application
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Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives - Options
- Supplier
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- Supplier Product No.
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 3.2596397 | ABIN2481412 | FACS ICC IF IHC IP WB | Atto 488 | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481415 | FACS ICC IF IHC IP WB | Atto 633 | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481416 | FACS ICC IF IHC IP WB | Atto 655 | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481417 | FACS ICC IF IHC IP WB | Atto 680 | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481421 | FACS ICC IF IHC IP WB | Biotin | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481420 | FACS ICC IF IHC IP WB | APC | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481422 | FACS ICC IF IHC IP WB | FITC | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481425 | FACS ICC IF IHC IP WB | PerCP | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481426 | FACS ICC IF IHC IP WB | PE | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481411 | FACS ICC IF IHC IP WB | Atto 390 | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481413 | FACS ICC IF IHC IP WB | Atto 565 | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481414 | FACS ICC IF IHC IP WB | Atto 594 | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481419 | FACS ICC IF IHC IP WB | Alkaline Phosphatase (AP) | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481424 | FACS ICC IF IHC IP WB | PE-Atto 594 | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481427 | FACS ICC IF IHC IP WB | Streptavidin | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481418 | FACS ICC IF IHC IP WB | Atto 700 | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 3.2596397 | ABIN2481423 | FACS ICC IF IHC IP WB | HRP | Mouse | IgG1 kappa | Log in to see | 8A7 | 0 | ||
| 1 | ABIN4320022 | FACS ICC IF IHC (p) WB | Mouse | IgG1 kappa | Log in to see | SPM252 | 0 | |||
| 1 | ABIN4320021 | FACS ICC IF IHC (p) WB | Mouse | IgG1 kappa | Log in to see | SPM252 | 0 | |||
| 1 | ABIN4320066 | FACS IF IHC (p) WB | Mouse | IgG1 kappa | Log in to see | HSPB1-774 | 0 |
General |
|
|---|---|
| Antigen | Heat Shock 27kDa Protein 1 (HSPB1) Antibodies |
| Reactivity | Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine) Alternatives |
| Host | Mouse Alternatives |
| Clonality (Clone) | |
| Conjugate | This HSPB1 antibody is un-conjugated Alternatives |
| Application |
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
|
|
9 references available |
| Supplier | Log in to see |
Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN361756) Images |
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| Specificity | Detects ~25 kDa or ~27 kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin. |
| Purification | Protein G Purified |
| Immunogen | HSP27 peptide |
| Clone | 8A7 |
| Isotype | IgG1 kappa |
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 antibody (ABIN361756) Images back to top |
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| Antigen | |
| Alternative Name | HSP27 (HSPB1 Antibody Abstract) |
| Background | HSP25 is the mouse homologue of the human HSP27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa(1). The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, HSP27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermo-tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (8). For more information visit our HSP27 Scientific Resource Guide at http://www.HSP27.com. |
| Gene ID | 3315 |
| NCBI Accession | NP_001532 |
| UniProt | P04792 |
| Pathways | MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling |
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 antibody (ABIN361756) Images back to top |
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| Application Notes |
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| Comment |
A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 μg of HeLa cell lysate by ECL immunoblot analysis. |
| Restrictions | For Research Use only |
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 antibody (ABIN361756) Images back to top |
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| Format | Liquid |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50 % glycerol, 0.09 % sodium azide |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Storage | -20 °C |
References for anti-HSPB1 antibody (ABIN361756)Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top |
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| Background publications |
Arrigo: "In search of the molecular mechanism by which small stress proteins counteract apoptosis during cellular differentiation." in: Journal of cellular biochemistry, Vol. 94, Issue 2, pp. 241-6, 2005 Van Montfort, Slingsby, Vierling: "Structure and function of the small heat shock protein/alpha-crystallin family of molecular chaperones." in: Advances in protein chemistry, Vol. 59, pp. 105-56, 2002 Jia, Ransom, Shibanuma, Liu, Welsh, Smoyer: "Identification and characterization of hic-5/ARA55 as an hsp27 binding protein." in: The Journal of biological chemistry, Vol. 276, Issue 43, pp. 39911-8, 2001 Sarto, Binz, Mocarelli: "Heat shock proteins in human cancer." in: Electrophoresis, Vol. 21, Issue 6, pp. 1218-26, 2000 Kim, Kim, Kim: "Crystal structure of a small heat-shock protein." in: Nature, Vol. 394, Issue 6693, pp. 595-9, 1998 Ehrnsperger, Gräber, Gaestel, Buchner: "Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation." in: The EMBO journal, Vol. 16, Issue 2, pp. 221-9, 1997 Welsh, Wu, Parvinen, Gilmont: "Variation in expression of hsp27 messenger ribonucleic acid during the cycle of the seminiferous epithelium and co-localization of hsp27 and microfilaments in Sertoli cells of the rat." in: Biology of reproduction, Vol. 55, Issue 1, pp. 141-51, 1997 Ciocca, Oesterreich, Chamness, McGuire, Fuqua: "Biological and clinical implications of heat shock protein 27,000 (Hsp27): a review." in: Journal of the National Cancer Institute, Vol. 85, Issue 19, pp. 1558-70, 1993 Welch: "Phorbol ester, calcium ionophore, or serum added to quiescent rat embryo fibroblast cells all result in the elevated phosphorylation of two 28,000-dalton mammalian stress proteins." in: The Journal of biological chemistry, Vol. 260, Issue 5, pp. 3058-62, 1985 |
ImagesProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN361756) back to top |
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| Supplier Images |
Hsp25 Hsp27 (8A7), somites of a rat embryo Courtesy of Mike Welsh, UMich .
SMC 114 Rat LungTissue 10ug Hsp25 WB 1 in 1000.
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