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|Antigen||Mitogen-Activated Protein Kinase 4 (MAPK4) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This MAPK4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-MAPK4 AntibodyTarget Details MAPK4 Application Details Handling References for anti-MAPK4 antibody (ABIN359633) Images
|Specificity||This antibody reacts to ERK4.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ERK4.|
Target Details MAPK4Product Details anti-MAPK4 Antibody Application Details Handling References for anti-MAPK4 antibody (ABIN359633) Images back to top
|Alternative Name||MAPK4 / ERK4 (MAPK4 Antibody Abstract)|
|Background||Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The CMGC group consists of 60 kinases including the cyclin-dependent kinase (CDK) and close relatives family, the MAP kinase (ERK) family, the glycogen synthase kinase 3 (GSK3) family, and the Cdc2-like kinase (CLK) family.Synonyms: Extracellular signal-regulated kinase 4, MAP kinase isoform p63, Mitogen-activated protein kinase 4, p63-MAPK|
|Gene ID||5596, 9606|
Application DetailsProduct Details anti-MAPK4 Antibody Target Details MAPK4 Handling References for anti-MAPK4 antibody (ABIN359633) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK4 Antibody Target Details MAPK4 Application Details References for anti-MAPK4 antibody (ABIN359633) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MAPK4 antibody (ABIN359633)Product Details anti-MAPK4 Antibody Target Details MAPK4 Application Details Handling Images back to top
Robinson, Cobb: "Mitogen-activated protein kinase pathways." in: Current opinion in cell biology, Vol. 9, Issue 2, pp. 180-6, 1997
Davis: "Transcriptional regulation by MAP kinases." in: Molecular reproduction and development, Vol. 42, Issue 4, pp. 459-67, 1996
Seger, Krebs: "The MAPK signaling cascade." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 9, Issue 9, pp. 726-35, 1995
Holland, Brown, Larsen: "Biotransformation of organic sulfides--IV. Formation of chiral benzyl alkyl and phenyl alkyl sulfoxides by Helminthosporium species NRRL 4671." in: Bioorganic & medicinal chemistry, Vol. 2, Issue 7, pp. 647-52, 1995
Zhu, Zhao, Moller, Flier: "Cloning and characterization of p97MAPK, a novel human homolog of rat ERK-3." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8202-11, 1994
ImagesProduct Details anti-MAPK4 Antibody Target Details MAPK4 Application Details Handling References for anti-MAPK4 antibody (ABIN359633) back to top