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|Antigen||Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies|
|Epitope||AA 337-363 Alternatives|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAPKAP Kinase 2 antibody is un-conjugated Alternatives|
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-MAPKAP Kinase 2 AntibodyTarget Details MAPKAP Kinase 2 Application Details Handling References for anti-MAPKAP Kinase 2 antibody (ABIN681523) Images
|Purification||Purified by Protein A.|
|Immunogen||KLH conjugated synthetic peptide derived from human MAPKAP Kinase 2|
Target Details MAPKAP Kinase 2Product Details anti-MAPKAP Kinase 2 Antibody Application Details Handling References for anti-MAPKAP Kinase 2 antibody (ABIN681523) Images back to top
|Alternative Name||Mapkap Kinase 2 (MAPKAPK2 Antibody Abstract)|
Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA, HSF1, HSP27/HSPB1, KRT18, KRT2, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA, PABPC1 and TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA and PARN, leading to stabilize GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
Subcellular location: Cytoplasm, Nucleus
Synonyms: MK2, MK-2, MAPKAP-K2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MAPKAPK2
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Handling References for anti-MAPKAP Kinase 2 antibody (ABIN681523) Images back to top
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details References for anti-MAPKAP Kinase 2 antibody (ABIN681523) Images back to top
|Buffer||Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.|
|Storage Comment||Store at -20°C|
|Expiry Date||12 months|
References for anti-MAPKAP Kinase 2 antibody (ABIN681523)Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Handling Images back to top
|Product cited in:||
Rosenzweig, Ou, Quinn: "P38 mitogen-activated protein kinase promotes dedifferentiation of primary articular chondrocytes in monolayer culture." in: Journal of cellular and molecular medicine, Vol. 17, Issue 4, pp. 508-17, 2013 Method employed by authors: Western Blotting (WB) (Sample species: Cow (Bovine)).
Rosenzweig, Djap, Ou, Quinn: "Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis." in: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, Vol. 20, Issue 12, pp. 1591-602, 2012 (1:500).
ImagesProduct Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Handling References for anti-MAPKAP Kinase 2 antibody (ABIN681523) back to top