anti-Monkey Chromogranin A antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Chromogranin A Antibody (supplied by: Log in to see )

Antigen
Chromogranin A (CHGA) Antibodies
  • fj05f09
  • si:dkey-177p2.2
  • wu:fj05f09
  • zgc:101749
  • zgc:56075
  • CHGA
  • ChrA
  • CGA
  • cgA
  • chromogranin A
  • uncharacterized CHGA
  • chga
  • CHGA
  • Chga
Reactivity
Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
1004
316
258
205
166
123
12
11
6
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
684
321
22
7
1
1
Clonality
Monoclonal
Conjugate
CF405M
40
35
31
31
27
16
16
16
15
15
15
15
15
15
15
15
15
15
14
14
14
14
14
14
14
14
14
14
14
14
14
13
12
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
682
632
460
405
306
211
211
196
168
102
16
13
13
8
7
3
2
1
1
Supplier
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Catalog No. ABIN6160675
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6160679 FACS IF IHC (f) CF®543 Mouse IgG Log in to see LK2H10 PHE5 CGA414 0
1 ABIN6160687 FACS IF IHC (f) CF®568 Mouse IgG Log in to see LK2H10 PHE5 CGA414 0
1 ABIN6160689 FACS IF IHC (f) CF®770 Mouse IgG Log in to see LK2H10 PHE5 CGA414 0
1 ABIN6160713 FACS IF IHC (f) CF405M Mouse IgG1 Log in to see LK2H10 PHE5 0
1 ABIN6160723 FACS IF IHC (f) CF®660R Mouse IgG1 Log in to see LK2H10 PHE5 0
1 ABIN6160725 FACS IF IHC (f) CF®568 Mouse IgG1 Log in to see LK2H10 PHE5 0
1 ABIN6160735 FACS IF IHC (f) CF®594 Mouse IgG1 Log in to see LK2H10 PHE5 0
1 ABIN6160733 FACS IF IHC (f) CF®488A Mouse IgG1 Log in to see LK2H10 PHE5 0
1 ABIN6160757 FACS IF IHC (f) CF®647 Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN6160755 FACS IF IHC (f) CF®543 Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN6160761 FACS IF IHC (f) CF®660R Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN6160765 FACS IF IHC (f) CF®770 Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN6160791 FACS IF IHC (f) CF®640R Mouse IgG1, kappa Log in to see PHE5 0
1 ABIN6160773 FACS IF IHC (f) CF®594 Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN6160797 FACS IF IHC (f) CF®555 Mouse IgG1, kappa Log in to see PHE5 0
1 ABIN6160809 FACS IF IHC (f) CF®488A Mouse IgG1, kappa Log in to see PHE5 0
1 ABIN6160673 FACS IF IHC (f) CF®405S Mouse IgG Log in to see LK2H10 PHE5 CGA414 0
1 ABIN6160677 FACS IF IHC (f) CF®640R Mouse IgG Log in to see LK2H10 PHE5 CGA414 0
1 ABIN6160681 FACS IF IHC (f) CF®647 Mouse IgG Log in to see LK2H10 PHE5 CGA414 0
1 ABIN6160683 FACS IF IHC (f) CF®555 Mouse IgG Log in to see LK2H10 PHE5 CGA414 0

General

Antigen Chromogranin A (CHGA) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(1004), (316), (258), (205), (166), (123), (12), (11), (6), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(684), (321), (22), (7), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate CF405M
(40), (35), (31), (31), (27), (16), (16), (16), (15), (15), (15), (15), (15), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (13), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(682), (632), (460), (405), (306), (211), (211), (196), (168), (102), (16), (13), (13), (8), (7), (3), (2), (1), (1)
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Product details

Target Details Application Details Handling Images
Purpose Mouse Monoclonal anti-Chromogranin A (LK2H10 + PHE5 + CGA414), CF405M Conjugate
Characteristics Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Human pheochromocytoma (LK2H10 & PHE5) & recombinant human chromogranin A protein (CGA414)
Clone LK2H10 PHE5 CGA414
Isotype IgG

Target Details

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Antigen
Alternative Name Chromogranin A (CHGA Antibody Abstract)
Background Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, Vasostatin I or II
Molecular Weight 68-75 kDa
Gene ID 1113, 150793
UniProt P10645
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

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Application Notes Immunohistology formalin-fixed 0.25-0.5 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Immunofluorescence 0.5-1 μg/mL
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Optimal dilution for a specific application should be determined by user
Comment

PC12 cells. Adrenal gland, bowel, thyroid, pancreas, or pheochromocytoma.

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light