anti-Mouse (Murine) CD40 antibody for Blocking Reagent

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • Bp50
  • CDW40
  • TNFRSF5
  • p50
  • AI326936
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • TNFSF5
  • CD40 molecule
  • CD40 antigen
  • CD40
  • Cd40
Reactivity
Mouse (Murine), Rat (Rattus)
634
273
76
22
15
10
9
6
4
4
3
2
2
2
1
1
1
1
Host
Armenian Hamster
479
194
161
22
6
1
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
78
78
46
35
18
14
12
11
10
9
6
6
6
6
6
6
6
6
6
6
6
5
5
4
4
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS)
531
252
206
181
112
94
82
80
50
32
19
16
13
9
6
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Options
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Pubmed
15 references available
Catalog No. ABIN1176841
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4 ABIN2658979 BR FACS IP IHC PE-Cy5 Rat IgG2a kappa Log in to see 3-23 3
1 ABIN2658980 BR FACS IP IHC PE-Cy5 Rat IgG2a kappa Log in to see 3-23 3

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(634), (273), (76), (22), (15), (10), (9), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Armenian Hamster
(479), (194), (161), (22), (6), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(78), (78), (46), (35), (18), (14), (12), (11), (10), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS)
(531), (252), (206), (181), (112), (94), (82), (80), (50), (32), (19), (16), (13), (9), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 15 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN1176841) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 ng/μg of protein.
Immunogen (BALB/c x NZB)F1 mouse-derived lymphoma WEHI-231
Clone HM40-3
Isotype IgM, kappa
Plasmids, Primers & others

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN1176841) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background The HM40-3 antibody reacts with CD40, a 40-50-kDa glycoprotein expressed on B lymphocytes and other antigen-presenting cells. The CD40 molecule has a central role in B-cell growth and differentiation. Furthermore, interactions of CD40 with its ligand, CD154, are involved in the initiation and effector stages of cell-mediated immune responses. CD40 may be involved in the triggering of NK cells and NK-T cells. Soluble HM40-3 antibody stimulates splenic and peritoneal B cells to proliferate in vitro. This antibody also induces spleen B cells to express the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). HM40-3 mAb has been demonstrated to inhibit the binding of soluble CD154 (gp39, CD40 Ligand) to soluble CD40 and to cell-surface CD40. This hamster mAb to a mouse leukocyte antigen has been observed to cross-react with similar populations of Lewis, Sprague-Dawley, and LOU16 rat leukocytes.
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN1176841) Images back to top
Application Notes For immunohistochemical staining of mouse tissue, we recommend the use of purified anti-mouse CD40 mAb 3/23 in our special formulation for immunohistochemistry, Cat. No. 550285. 553721 Note: This product may appear to contain aggregation and/or precipitation of the IgM antibody. Investigators are advised to briefly spin down any particulate matter.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN1176841) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm filtered.
Preservative Azide free
Storage 4 °C
Storage Comment This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store undiluted at 4°C.

References for anti-CD40 antibody (ABIN1176841)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Product cited in:

Turner, Rakhmilevich, Burdelya, Neal, Imboden, Sondel, Yu: "Anti-CD40 antibody induces antitumor and antimetastatic effects: the role of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 89-94, 2001

Trinité, Voisine, Yagita, Josien: "A subset of cytolytic dendritic cells in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 8, pp. 4202-8, 2000

Leifeld, Trautwein, Dumoulin, Manns, Sauerbruch, Spengler: "Enhanced expression of CD80 (B7-1), CD86 (B7-2), and CD40 and their ligands CD28 and CD154 in fulminant hepatic failure." in: The American journal of pathology, Vol. 154, Issue 6, pp. 1711-20, 1999

Akiba, Oshima, Takeda, Atsuta, Nakano, Nakajima, Nohara, Yagita, Okumura: "CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7058-66, 1999

Tomura, Yu, Ahn, Yamashita, Yang, Ono, Hamaoka, Kawano, Taniguchi, Koezuka, Fujiwara: "A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 93-101, 1999

Grewal, Flavell: "CD40 and CD154 in cell-mediated immunity." in: Annual review of immunology, Vol. 16, pp. 111-35, 1998

Ridge, Di Rosa, Matzinger: "A conditioned dendritic cell can be a temporal bridge between a CD4+ T-helper and a T-killer cell." in: Nature, Vol. 393, Issue 6684, pp. 474-8, 1998

Kaneko, Hirose, Abe, Yagita, Okumura, Shirai: "CD40-mediated stimulation of B1 and B2 cells: implication in autoantibody production in murine lupus." in: European journal of immunology, Vol. 26, Issue 12, pp. 3061-5, 1997

Kawano, Cui, Koezuka, Toura, Kaneko, Motoki, Ueno, Nakagawa, Sato, Kondo, Koseki, Taniguchi: "CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1626-9, 1997

Kashiwada, Kaneko, Yagita, Okumura, Takemori: "Activation of mitogen-activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells." in: European journal of immunology, Vol. 26, Issue 7, pp. 1451-8, 1996

Inaba, Inaba, Fukuba, Mori, Haruna, Doi, Adachi, Iwai, Hosaka, Hisha: "Activation of thymic B cells by signals of CD40 molecules plus interleukin-10." in: European journal of immunology, Vol. 25, Issue 5, pp. 1244-8, 1995

Foy, Laman, Ledbetter, Aruffo, Claassen, Noelle: "gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory." in: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 157-63, 1994

Inaba, Witmer-Pack, Inaba, Hathcock, Sakuta, Azuma, Yagita, Okumura, Linsley, Ikehara, Muramatsu, Hodes, Steinman: "The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1849-60, 1994

Parry, Hasbold, Holman, Klaus: "Hypercross-linking surface IgM or IgD receptors on mature B cells induces apoptosis that is reversed by costimulation with IL-4 and anti-CD40." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2821-9, 1994

Noelle, Ledbetter, Aruffo: "CD40 and its ligand, an essential ligand-receptor pair for thymus-dependent B-cell activation." in: Immunology today, Vol. 13, Issue 11, pp. 431-3, 1993