anti-Mouse (Murine) CD40 antibody for Western Blotting

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • Bp50
  • CDW40
  • TNFRSF5
  • p50
  • AI326936
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • TNFSF5
  • CD40 molecule
  • CD40 antigen
  • CD40
  • Cd40
Epitope
AA 22-36, N-Term
54
44
34
24
23
22
20
16
12
8
7
6
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
686
321
74
20
15
10
9
6
4
4
3
2
2
1
1
1
1
1
Host
Rabbit
532
211
189
22
7
4
Clonality
Polyclonal
Conjugate
This CD40 antibody is un-conjugated
90
88
50
41
24
21
17
15
15
14
9
9
9
9
9
9
9
8
7
7
7
7
6
6
6
6
5
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
616
277
223
223
112
103
97
85
49
33
20
19
13
9
8
6
6
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN3043683
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.8412423 ABIN222919 WB Rabbit IgG Log in to see Polyclonal 1
2.8412423 ABIN2704615 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
2.8412423 ABIN1531702 ELISA WB Rabbit IgG AA 220-269, pThr254 Log in to see Polyclonal 1
2.8412423 ABIN1533424 IHC ELISA WB Rabbit IgG AA 228-277 Log in to see Polyclonal 0
2.8412423 ABIN4886526 ELISA IHC (p) WB Rabbit IgG AA 20-193 Log in to see Polyclonal 0
2.8412423 ABIN1871634 WB Rabbit IgG Log in to see Polyclonal 0
2.8412423 ABIN1584207 ELISA WB Rabbit N-Term Log in to see Polyclonal 1
2.8412423 ABIN3182275 ELISA WB Rabbit IgG pThr254 Log in to see Polyclonal 0
2.8412423 ABIN3183772 ELISA IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
2.8412423 ABIN1106352 WB Rabbit IgG N-Term Log in to see Polyclonal 0
2.8412423 ABIN1871710 ICC IHC WB Rabbit IgG AA 26-193 Log in to see Polyclonal 0
2.8412423 ABIN3020704 WB Rabbit IgG C-Term Log in to see Polyclonal 0
2.8412423 ABIN3030396 WB Rabbit IgG N-Term Log in to see Polyclonal 0
2.8412423 ABIN2988454 WB Rabbit IgG C-Term Log in to see Polyclonal 0
2.8412423 ABIN5960844 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
2.8412423 ABIN5693201 ELISA WB Rabbit AA 24-193 Log in to see Polyclonal 0
2.8412423 ABIN1848816 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 0
2.8412423 ABIN2904422 IF/ICC IHC IP WB Rabbit AA 26-193 Log in to see Polyclonal 0
1 ABIN4899798 CyTOF ELISA (Capture) FACS WB Rat IgG1 AA 24-193 Log in to see 65924 1
1 ABIN4293028 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope AA 22-36, N-Term
(54), (44), (34), (24), (23), (22), (20), (16), (12), (8), (7), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(686), (321), (74), (20), (15), (10), (9), (6), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(532), (211), (189), (22), (7), (4)
Clonality
Polyclonal
Conjugate This CD40 antibody is un-conjugated
(90), (88), (50), (41), (24), (21), (17), (15), (15), (14), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(616), (277), (223), (223), (112), (103), (97), (85), (49), (33), (20), (19), (13), (9), (8), (6), (6), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN3043683) Images
Purpose Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 5(CD40) detection. Tested with WB in Mouse.
Sequence QCVTCSDKQY LHDGQ
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 5(CD40) detection. Tested with WB in Mouse.
Gene Name: CD40 Molecule, TNF receptor superfamily member 5
Protein Name: Tumor necrosis factor receptor superfamily member 5
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of mouse CD40(22-36aa, QCVTCSDKQYLHDGQ).
Isotype IgG
Plasmids, Primers & others

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN3043683) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor(TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand(CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.

Synonyms: AI326936 antibody|B cell associated molecule CD40 antibody|B cell surface antigen CD40 antibody|B cell-associated molecule antibody|B-cell surface antigen CD40 antibody|Bp50 antibody|CD 40 antibody|CD40 antibody|CD40 antigen(TNF receptor superfamily member 5) antibody|CD40 antigen antibody|CD40 Molecule antibody|CD40 Molecule, TNF receptor superfamily member 5 antibody|CD40 protein antibody|CD40 type II isoform antibody|CD40L receptor antibody|CDw40 antibody|GP39 antibody|HIGM1 antibody|IGM antibody|IMD3 antibody|MGC9013 antibody|Nerve growth factor receptor related B lymphocyte activation molecule antibody|OTTHUMP00000031699 antibody|OTTHUMP00000031700 antibody|p50 antibody|T-BAM antibody|TBAM antibody|TNF receptor superfamily member 5 antibody|TNFRSF5 antibody|TNR5_HUMAN antibody|TRAP antibody|Tumor necrosis factor receptor superfamily , member 5 antibody|Tumor necrosis factor receptor superfamily member 5 antibody|Tumor necrosis factor receptor superfamily member 5 precursor antibody|Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
UniProt P27512
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN3043683) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN3043683) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-CD40 antibody (ABIN3043683)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Product cited in:

Meng, Li, Geng, Ohe, Yu, E, Ye, Yang, Kato, Zhang, Ishida, Ohta, Jin, Kakehata, Geng, Yamakawa: "Decreased expression of follicular dendritic cell-secreted protein correlates with increased immunoglobulin A production in the tonsils of individuals with immunoglobulin A nephropathy." in: Translational research : the journal of laboratory and clinical medicine, Vol. 166, Issue 3, pp. 281-91, 2015

Peng, Cheng, Zeng, Shi, Bao, Mao, Ji, Li, Guo, Liang, Wu: "The role of RANTES as a crucial downstream cytokine in calcineurin-dependent VSMC apoptosis stimulated by INFgamma and CD40L." in: Cell biology international, Vol. 34, Issue 5, pp. 447-53, 2010

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN3043683) back to top
Supplier Images
Western Blotting (WB) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 22-36), (N-Term) antibody (ABIN3043683) Anti-CD40 antibody, Western blotting Lane 1: Recombinant Mouse CD 40 Protein 10ng Lan...
Western Blotting (WB) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 22-36), (N-Term) antibody (ABIN3043683) Anti-CD40 antibody, Western blotting Lane 1: Recombinant Human CD40 Protein 10ng Lan...
Western Blotting (WB) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 22-36), (N-Term) antibody (ABIN3043683) Anti-CD40 antibody, Western blotting Lane 1: Recombinant Mouse CD 40 Protein 10ng Lan...