anti-Human EGFR antibody for ELISA (Detection)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
AA 25-645
147
134
64
64
60
58
57
54
54
53
49
49
43
39
36
33
32
30
29
28
27
26
26
25
24
22
19
18
18
17
16
16
16
15
15
15
15
15
14
14
13
11
10
9
9
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2444
866
700
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Goat
1462
868
92
44
10
3
2
1
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is conjugated to Biotin
102
80
71
64
58
56
34
34
28
28
28
28
28
28
28
27
27
26
26
26
24
21
21
21
21
21
20
19
18
4
2
2
1
Application
ELISA (Detection), Flow Cytometry (FACS), Western Blotting (WB)
1451
701
615
556
511
272
260
230
127
84
66
56
45
24
19
14
12
11
7
5
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN4899924
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1995319 ELISA ELISA (Detection) IF IF/ICC IP WB Rabbit IgG AA 1-645 Log in to see 021
1 ABIN5634569 ELISA (Detection) FACS WB Biotin Goat IgG AA 25-645 Log in to see Polyclonal

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 25-645
(147), (134), (64), (64), (60), (58), (57), (54), (54), (53), (49), (49), (43), (39), (36), (33), (32), (30), (29), (28), (27), (26), (26), (25), (24), (22), (19), (18), (18), (17), (16), (16), (16), (15), (15), (15), (15), (15), (14), (14), (13), (11), (10), (9), (9), (8), (8), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2444), (866), (700), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Goat
(1462), (868), (92), (44), (10), (3), (2), (1), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is conjugated to Biotin
(102), (80), (71), (64), (58), (56), (34), (34), (28), (28), (28), (28), (28), (28), (28), (27), (27), (26), (26), (26), (24), (21), (21), (21), (21), (21), (20), (19), (18), (4), (2), (2), (1)
Application ELISA (Detection), Flow Cytometry (FACS), Western Blotting (WB)
(1451), (701), (615), (556), (511), (272), (260), (230), (127), (84), (66), (56), (45), (24), (19), (14), (12), (11), (7), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN4899924) Images
Specificity Detects human EGFR in ELISAs and Western blots. In ELISAs, this antibody shows less than 3 % cross-reactivity with recombinant mouse EGFR and less than 0.1 % cross-reactivity with recombinant human (rh) EGF, rhErbB2, and rhErbB3.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human EGFR, Leu25-Ser645, Accession # CAA25240
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR antibody (ABIN4899924) Images back to top
Antigen
Alternative Name EGF R/ErbB1 (EGFR Antibody Abstract)
Background Alternative Names: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGF R, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Gene ID 1956
Research Area Receptors, Growth Factors, Hormones, Cancer, Tyrosine Kinases
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR antibody (ABIN4899924) Images back to top
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
ELISA Detection (Matched Antibody Pair) 0.1-0.4 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR antibody (ABIN4899924) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EGFR antibody (ABIN4899924)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Sakanyan, Hulin, Alves de Sousa, Silva, Hambardzumyan, Nedellec, Tomasoni, Logé, Pineau, Roussakis, Fleury, Artaud: "Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1." in: Scientific reports, Vol. 6, pp. 21088, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Thompson, Bloom, Ogiue-Ikeda, Machida: "SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR." in: BMC biotechnology, Vol. 15, pp. 60, 2015 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Bryan, Regan, Pirrie, Devall, Cheng, Zeegers, James, Knowles, Ward: "Protein shedding in urothelial bladder cancer: prognostic implications of soluble urinary EGFR and EpCAM." in: British journal of cancer, Vol. 112, Issue 6, pp. 1052-8, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Gonzalez, Seurynck-Servoss, Crowley, Brown, Omenn, Hayes, Zangar: "Development and validation of sandwich ELISA microarrays with minimal assay interference." in: Journal of proteome research, Vol. 7, Issue 6, pp. 2406-14, 2008 (Sample species: Human).

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN4899924) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 25-645) antibody (Biotin) (ABIN4899924) Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry. A431 human epithe...
Flow Cytometry (FACS) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 25-645) antibody (Biotin) (ABIN4899924) Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry. A431 human epithe...