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|Antigen||Epidermal Growth Factor Receptor (EGFR) Antibodies|
|Epitope||AA 25-645 Alternatives|
|Conjugate||This EGFR antibody is conjugated to Biotin Alternatives|
ELISA (Detection), Flow Cytometry (FACS), Western Blotting (WB)
|4 references available|
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Product Details anti-EGFR AntibodyTarget Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN4899924) Images
|Specificity||Detects human EGFR in ELISAs and Western blots. In ELISAs, this antibody shows less than 3 % cross-reactivity with recombinant mouse EGFR and less than 0.1 % cross-reactivity with recombinant human (rh) EGF, rhErbB2, and rhErbB3.|
|Sterility||0.2 μm filtered|
|Immunogen||Mouse myeloma cell line NS0-derived recombinant human EGFR, Leu25-Ser645, Accession # CAA25240|
Target Details EGFRProduct Details anti-EGFR Antibody Application Details Handling References for anti-EGFR antibody (ABIN4899924) Images back to top
|Alternative Name||EGF R/ErbB1 (EGFR Antibody Abstract)|
|Background||Alternative Names: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 126.96.36.199, EGF R, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1|
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation|
Application DetailsProduct Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR antibody (ABIN4899924) Images back to top
Western Blot 0.1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
ELISA Detection (Matched Antibody Pair) 0.1-0.4 μg/mL
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR antibody (ABIN4899924) Images back to top
|Reconstitution||Reconstitute at 0.2 mg/mL in sterile PBS.|
|Buffer||Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.|
|Storage||4 °C,-20 °C,-80 °C|
|Storage Comment||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.|
|Expiry Date||12 months|
References for anti-EGFR antibody (ABIN4899924)Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
|Product cited in:||
Sakanyan, Hulin, Alves de Sousa, Silva, Hambardzumyan, Nedellec, Tomasoni, Logé, Pineau, Roussakis, Fleury, Artaud: "Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1." in: Scientific reports, Vol. 6, pp. 21088, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).
Thompson, Bloom, Ogiue-Ikeda, Machida: "SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR." in: BMC biotechnology, Vol. 15, pp. 60, 2015 Method employed by authors: Functional Studies (Func), Functional Studies (Func) (Sample species: Human).
Bryan, Regan, Pirrie, Devall, Cheng, Zeegers, James, Knowles, Ward: "Protein shedding in urothelial bladder cancer: prognostic implications of soluble urinary EGFR and EpCAM." in: British journal of cancer, Vol. 112, Issue 6, pp. 1052-8, 2015 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).
Gonzalez, Seurynck-Servoss, Crowley, Brown, Omenn, Hayes, Zangar: "Development and validation of sandwich ELISA microarrays with minimal assay interference." in: Journal of proteome research, Vol. 7, Issue 6, pp. 2406-14, 2008 (Sample species: Human).
ImagesProduct Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN4899924) back to top