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|Antigen||Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) Antibodies|
|Reactivity||Bat, Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio) Alternatives|
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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|Specificity||Human NR1H3 / LXR Alpha|
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Goat, Pig, Guinea pig, Chicken, Zebrafish (100%).|
Synthetic peptide from C-Terminus of human NR1H3 (Q13133, NP_005684). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Salmon, Stickleback, Zebrafish (100%).
Type of Immunogen: Synthetic peptide
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|Alternative Name||NR1H3 / LXR Alpha (NR1H3 Antibody Abstract)|
Name/Gene ID: NR1H3
Subfamily: NR1 Thyroid hormone-like
Synonyms: NR1H3, Liver X receptor alpha, LXRA, LXR-a, Oxysterols receptor LXR-alpha, LXR alpha, Lxralpha, RLD-1
|Pathways||Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Nuclear Hormone Receptor Binding, Cellular Response to Molecule of Bacterial Origin, Hepatitis C|
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Target Species of Antibody: Human
|Restrictions||For Research Use only|
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|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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