anti-Cow (Bovine) Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) antibody for Western Blotting

Recommended Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) Antibody

Antigen
Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) Antibodies
  • GR
  • gcr
  • grl
  • xGR
  • gccr
  • gr
  • zgc:113038
  • NR3C1
  • GCL
  • LOC100302444
  • Gcr
  • Grl
  • Grl-1
  • Grl1
  • GRL
  • GCCR
  • GCR
  • NO
  • GR-A
  • nr3c1
  • nuclear receptor subfamily 3 group C member 1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1
  • glucocorticoid receptor
  • NR3C1
  • nr3c1
  • LOC100302444
  • Nr3c1
Epitope
N-Term
26
23
20
18
17
16
12
10
10
9
7
7
7
6
5
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
290
150
134
7
7
5
5
5
4
3
2
1
1
1
Host
Rabbit
249
57
5
Clonality
Polyclonal
Conjugate
Un-conjugated
23
17
10
7
6
6
6
6
6
6
6
6
3
3
3
3
3
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
225
119
105
63
53
39
35
29
27
17
4
3
2
1
1
Options

Available images

Catalog No. ABIN2782262
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
9.446512 ABIN152763 ChIP ICC IF IHC (p) IHC WB Rabbit IgG Polyclonal 1
7.890442 ABIN2706239 IHC WB Rabbit pSer211 Polyclonal 0
7.890442 ABIN2777208 IHC WB Rabbit N-Term Polyclonal 0
7.890442 ABIN2971701 IHC WB Rabbit Center, pSer211 Polyclonal 0
4 ABIN6748602 IHC IHC (p) WB Rabbit AA 180-229 Polyclonal 0
1 ABIN2743324 IHC IHC (p) WB Rabbit pSer211 Polyclonal 0
1 ABIN1918434 IF IHC IHC (p) IP WB Rabbit IgG AA 150-200 Polyclonal 0

General

Antigen Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) Antibodies
Epitope N-Term
(26), (23), (20), (18), (17), (16), (12), (10), (10), (9), (7), (7), (7), (6), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(290), (150), (134), (7), (7), (5), (5), (5), (4), (3), (2), (1), (1), (1)
Host Rabbit
(249), (57), (5)
Clonality
Polyclonal
Conjugate Un-conjugated
(23), (17), (10), (7), (6), (6), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (3), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(225), (119), (105), (63), (53), (39), (35), (29), (27), (17), (4), (3), (2), (1), (1)
Pubmed 1 reference available
Supplier

Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN980920
Sequence NVKLYTTDQS TFDILQDLEF SSGSPGKETN ESPWRSDLLI DENCLLSPLA
Predicted Reactivity Cow: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rat: 93%
Characteristics This is a rabbit polyclonal antibody against NR3C1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human NR3C1

Target Details

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Antigen
Alternative Name NR3C1 (NR3C1 Antibody Abstract)
Background NR3C1 is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined.
Alias Symbols: GCCR, GCR, GR, GRL
Protein Interaction Partner: TTC5, HSP90AA1, TXN2, UBC, TXN, FKBP5, DCAF6, RABEP2, BRCC3, LARP7, GAPVD1, CD2AP, SF3A1, HNRNPR, TRIM28, HDAC1, MTA2, CCDC6, CENPB, TMF1, PPARG, STUB1, BAG1, RPS6KA5, NCOA1, POLR1D, NCOA2, SRA1, PRKDC, RAD54L2, MAGEA11, PPP5C, PPID, GNB2, GNB1, FKBP4, GN
Protein Size: 777
Molecular Weight 86 kDa
Gene ID 2908
NCBI Accession NM_000176, NP_000167
UniProt P04150
Pathways Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 777 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Yoshikawa, Shimizu, Sano, Ohnuma, Iwata, Hosono, Fukuda, Morimoto, Tanaka: "Role of the hinge region of glucocorticoid receptor for HEXIM1-mediated transcriptional repression." in: Biochemical and biophysical research communications, Vol. 371, Issue 1, pp. 44-9, 2008

Images

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Supplier Images
Western Blotting (WB) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (N-Term) antibody (ABIN2782262) WB Suggested Anti-NR3C1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posi...
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (N-Term) antibody (ABIN2782262) Immunohistochemistry with Colon, submucosal plexus tissue at an antibody concentratio...
Western Blotting (WB) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (N-Term) antibody (ABIN2782262) Host: Rabbit Target Name: NR3C1 Sample Type: Hela Antibody Dilution: 1.0ug/ml