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|Antigen||Splicing Factor 1 (SF1) Antibodies|
|Epitope||AA 517-566 Alternatives|
|Reactivity||Bat, Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This Splicing Factor 1 antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Splicing Factor 1 AntibodyTarget Details Splicing Factor 1 Application Details Handling Images
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%).|
Synthetic peptide located between aa517-566 of human SF1 (Q15637, NP_004621). Percent identity by BLAST analysis: Human, Chimpanzee, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Armadillo (100%), Gibbon, Slime mold (92%).
Type of Immunogen: Synthetic peptide
|Plasmids, Primers & others|
Target Details Splicing Factor 1Product Details anti-Splicing Factor 1 Antibody Application Details Handling Images back to top
|Alternative Name||SF1 (SF1 Antibody Abstract)|
Name/Gene ID: SF1
Synonyms: SF1, D11S636, MBBP, ZCCHC25, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, BBP, ZNF162, Splicing factor 1, ZFM1
|Pathways||Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location|
Application DetailsProduct Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Handling Images back to top
Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
Target Species of Antibody: Human
|Restrictions||For Research Use only|
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|Reconstitution||After adding water, will consist of PBS buffer with 2 % sucrose|
|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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