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|Antigen||Checkpoint Kinase 1 (CHEK1) Antibodies|
|Epitope||AA 1-474 Alternatives|
|Reactivity||Human, Monkey, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CHEK1 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-CHEK1 AntibodyTarget Details CHEK1 Application Details Handling Images
|Specificity||This antibody recognizes CHK1 kinase.|
|Immunogen||A GST fusion protein corresponding to amino acid sequence 1-474 with having a molecular weight of 54 kD.|
Target Details CHEK1Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
|Alternative Name||CHK1 (CHEK1 Antibody Abstract)|
|Background||One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic-dependent kinase cdc2, which regulates entry in to mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of cdc2. It is hypothesized that chk 1 induces serine 216 phosphorylation of Cdc 25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating cdc2.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1|
|Pathways||p53 Signaling, Apoptosis, Cell Division Cycle|
Application DetailsProduct Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Western Blot: 1-10 μg/mL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
|Buffer||Phosphate buffered saline with 0.08 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
ImagesProduct Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top