anti-Mouse (Murine) CHEK1 antibody for Western Blotting

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CAMK/CAMKL/CHK1 protein kinase Chk1
  • checkpoint kinase 1 S homeolog
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1.S
  • Chek1
  • chek1
Epitope
Internal Region, pSer345
74
57
40
36
28
17
16
15
14
12
7
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
513
211
180
14
14
10
10
10
7
5
5
5
4
2
1
1
Host
Rabbit
453
54
22
7
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
14
13
12
11
10
8
3
3
3
3
3
3
3
3
3
3
3
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
446
221
172
80
70
40
32
21
21
16
13
7
6
6
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Catalog No. ABIN2857048
€ 412.55
Plus shipping costs €45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.563175 ABIN969476 ELISA WB Mouse IgG1 Log in to see 2G1D5 2
24.306385 ABIN6255201 ELISA ICC IF IHC WB Rabbit IgG pSer317 Log in to see Polyclonal 1
24.306385 ABIN6255160 ELISA ICC IF IHC WB Rabbit IgG pSer280 Log in to see Polyclonal 1
20.063175 ABIN3209997 ELISA WB Mouse IgG1 Log in to see 2G1D5 0
20.063175 ABIN634171 IHC WB Rabbit Log in to see Polyclonal 0
19.806385 ABIN2779737 IHC WB Rabbit Middle Region Log in to see Polyclonal 1
19.806385 ABIN6256251 ELISA ICC IF IHC WB Rabbit IgG pSer296 Log in to see Polyclonal 0
19.806385 ABIN6256255 ELISA ICC IF IHC WB Rabbit IgG pSer301 Log in to see Polyclonal 0
19.806385 ABIN6255167 ELISA ICC IF IHC WB Rabbit IgG pSer286 Log in to see Polyclonal 0
17.063175 ABIN3181961 ELISA IHC WB Rabbit IgG pSer317 Log in to see Polyclonal 0
17.063175 ABIN3182434 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal 0
17.063175 ABIN3183900 ELISA IF WB Rabbit IgG Ser2481 Log in to see Polyclonal 0
17.063175 ABIN3183903 ELISA IHC WB Rabbit IgG Ser249 Log in to see Polyclonal 0
17.063175 ABIN3183901 ELISA IF IHC WB Rabbit IgG Ser2484 Log in to see Polyclonal 0
17.063175 ABIN634170 WB Rabbit Log in to see Polyclonal 0
14.5 ABIN6260808 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 1
13.806385 ABIN6260806 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
13.806385 ABIN3049475 IHC WB Rabbit IgG Log in to see Polyclonal 0
13.25679 ABIN4901262 ELISA WB Mouse IgG1 Log in to see 2G1D5 0
13.25679 ABIN1842465 IHC WB Rabbit IgG pSer317 Log in to see Polyclonal 0

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope Internal Region, pSer345
(74), (57), (40), (36), (28), (17), (16), (15), (14), (12), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(513), (211), (180), (14), (14), (10), (10), (10), (7), (5), (5), (5), (4), (2), (1), (1)
Host Rabbit
(453), (54), (22), (7)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(14), (13), (12), (11), (10), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(446), (221), (172), (80), (70), (40), (32), (21), (21), (16), (13), (7), (6), (6)
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling Images
Cross-Reactivity Cow (Bovine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
Cross-Reactivity (Details) Rat (100 %), Zebrafish (88 %), Xenopus laevis (88 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to Chk1 (phospho Ser345) (CHK1 checkpoint homolog (S. pombe))
Chk1 (phospho Ser345) antibody [C1C2], Internal
Purification Purified by antigen-affinity chromatography.
Isotype IgG
Plasmids, Primers & others

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Cellular Localization: Nucleus , Cytoplasm
Molecular Weight 54 kDa
Gene ID 1111
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: HCT116 , HCT116 cells with 30uM cisplatin treatment for 24hr

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) ICC/IF Image Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Se...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) IHC-P Image Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using C...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) WB Image Sample: 20 ug of HCT116 P53 whole cell lysate 10% SDS PAGE Chk1-phopho-S345 ...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) IHC-P Image Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Ser...